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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
06/10/2022 |
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Actualizado : |
06/10/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HANNAH BULLOCK, Synergy America Inc., Atlanta, GA 30329, USA; MARINA MAURENTE BERON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; NÉLIDA RODRÍGUEZ-OSORIO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; LUCIANA SILVA-FLANNERY, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS CABRERA, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay; RODRIGO PUENTES, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; JANA M. RITTER, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay. |
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Título : |
Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 |
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ISSN : |
1999-4915 |
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DOI : |
10.3390/v14092042 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com --
Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock |
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Contenido : |
ABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. MenosABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus mor... Presentar Todo |
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Palabras claves : |
Abortion; Cattle; Emerging diseases; Epsilonpolyomavirus bovis; Nephropathy; Next generation sequencing; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Polyomavirus; Reproductive diseases; Viral diseases. |
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Asunto categoría : |
L20 Ecología animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16810/1/viruses-14-02042-v2.pdf
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Marc : |
LEADER 04533naa a2200445 a 4500
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008 2022 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v14092042$2DOI
100 1 $aGIANNITTI, F.
245 $aBovine Polyomavirus-1 (Epsilonpolyomavirus bovis)$bAn emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022. Academic Editors: Fernando Bauermann and Mayara Maggioli. Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com -- Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock
520 $aABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors.
653 $aAbortion
653 $aCattle
653 $aEmerging diseases
653 $aEpsilonpolyomavirus bovis
653 $aNephropathy
653 $aNext generation sequencing
653 $aPathology
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPolyomavirus
653 $aReproductive diseases
653 $aViral diseases
700 1 $aSILVEIRA, C.S.
700 1 $aBULLOCK, H.
700 1 $aBERON, M.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRODRÍGUEZ-OSORIO, N.
700 1 $aSILVA-FLANNERY, L.
700 1 $aPERDOMO, T.
700 1 $aCABRERA, A.
700 1 $aPUENTES, R.
700 1 $aCOLINA, R.
700 1 $aRITTER, J.M.
700 1 $aCASTELLS, M.
773 $tViruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
20/03/2023 |
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Actualizado : |
20/03/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CIAPPESONI, G.; DE BARBIERI, I.; MONTOSSI, F.; RAMOS, Z.; GIMENO, D.; VERA, B.; GIONANNINI, N. |
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Afiliación : |
CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ZULLY MARGOT RAMOS ALVEZ, Massey University, Palmerston North, New Zealand; DIEGO GIMENO, SUL, Montevideo, Uruguay; BRENDA VERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. GIONANNINI, INTA, Bariloche, Argentina. |
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Título : |
PL-3 Pass, present and future of Merino in Argentina and Uruguay. [conference abstract]. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Animal - science proceedings, March 2023, Volume 14, Issue 1, Pages 3-5. doi: https://doi.org/10.1016/j.anscip.2023.01.004 |
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ISSN : |
2772-283X |
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DOI : |
10.1016/j.anscip.2023.01.004 |
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Idioma : |
Inglés |
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Notas : |
Article history: Available online 13 March 2023, Version of Record 13 March 2023. -- Corresponding author: Gabriel Ciappesoni Scarone. E-mail: gciappesoni@inia.org.uy --
Animal - science proceedings Volume 14, Contents: 10th International Sheep Veterinary Congress (ISVC 2023). |
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Contenido : |
Merino breed in Uruguay: The Merino breed was introduced to Uruguay in the 19th century (1875) when a family of Basque-French immigrants arrived in the country with their flock. In 1901, the first pedigree imports were recorded in the Rural Association of Uruguay (ARU, 2021). Imports of pedigree animals from Argentina began in 1903, in 1911 from the USA and in 1924 the first rams were imported from Australia. The Australian variety was imported to Uruguay through two English companies based in Argentine Patagonia. In its beginnings, the Merino breed was used to cross with animals of thick wool breeds such as Lincoln and Romney Marsh, producing the renowned "Río de la Plata wool". In 1943, the SCMAU was founded, its first president being Dr. Domingo Bordaberry. Since that date, it has had uninterrupted growth, going from 4% to more than 38% of the national stock today (ARU, 2021). |
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Palabras claves : |
Animal breeding; Australian Merino; Valorisation; Wool. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.sciencedirect.com/sdfe/reader/pii/S2772283X23000043/pdf
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Marc : |
LEADER 01993nam a2200265 a 4500
001 1063984
005 2023-03-20
008 2023 bl uuuu u01u1 u #d
022 $a2772-283X
024 7 $a10.1016/j.anscip.2023.01.004$2DOI
100 1 $aCIAPPESONI, G.
245 $aPL-3 Pass, present and future of Merino in Argentina and Uruguay. [conference abstract].$h[electronic resource]
260 $aAnimal - science proceedings, March 2023, Volume 14, Issue 1, Pages 3-5. doi: https://doi.org/10.1016/j.anscip.2023.01.004$c2023
500 $aArticle history: Available online 13 March 2023, Version of Record 13 March 2023. -- Corresponding author: Gabriel Ciappesoni Scarone. E-mail: gciappesoni@inia.org.uy -- Animal - science proceedings Volume 14, Contents: 10th International Sheep Veterinary Congress (ISVC 2023).
520 $aMerino breed in Uruguay: The Merino breed was introduced to Uruguay in the 19th century (1875) when a family of Basque-French immigrants arrived in the country with their flock. In 1901, the first pedigree imports were recorded in the Rural Association of Uruguay (ARU, 2021). Imports of pedigree animals from Argentina began in 1903, in 1911 from the USA and in 1924 the first rams were imported from Australia. The Australian variety was imported to Uruguay through two English companies based in Argentine Patagonia. In its beginnings, the Merino breed was used to cross with animals of thick wool breeds such as Lincoln and Romney Marsh, producing the renowned "Río de la Plata wool". In 1943, the SCMAU was founded, its first president being Dr. Domingo Bordaberry. Since that date, it has had uninterrupted growth, going from 4% to more than 38% of the national stock today (ARU, 2021).
653 $aAnimal breeding
653 $aAustralian Merino
653 $aValorisation
653 $aWool
700 1 $aDE BARBIERI, I.
700 1 $aMONTOSSI, F.
700 1 $aRAMOS, Z.
700 1 $aGIMENO, D.
700 1 $aVERA, B.
700 1 $aGIONANNINI, N.
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